Abstract
Objective:
To investigate the combined effects of Crila and Green tea extract, epigallocatechin gallate, (EGCG) compared to single treatments on human uterine fibroid cells.
Design:
Human uterine leiomyoma (HuLM) cells were treated with different concentrations of Crila, alone or in combination with EGCG and several experiments were employed.
Setting:
Laboratory study PATIENTS(S): N/A.
Intervention(s):
Crila, EGCG MAIN OUTCOME MEASURE(S): Cell proliferation assay, drug synergy using combination index, protein and gene expression analysis of proliferation marker PCNA and apoptosis marker BAX using western blotting and qPCR, respectively.
Results:
results showed that tested Crila concentrations, when combined with 25 and 50 μM EGCG, exerted synergistic growth inhibitory effects on HuLM viability. This inhibitory effect on HuLM cell viability was due to decreased cell proliferation as shown by a decrease of the proliferation marker PCNA at mRNA and protein levels, rather than inducing apoptosis.
Conclusion:
Our study conclude that the utility of natural compounds may provide a safe and cost-effective alternative to currently used short-term hormonal therapies against UFs.
Full text
