Abstract
Scope:
Salmonella is the main food-borne pathogen, which can infect intestinal epithelial cells and cause colitis. Genistein had a variety of biological activities that alleviated colitis induced by sodium dextran sulfate in a variety of ways, but its protective effects on colitis caused by pathogenic bacteria are still unknown.
Methods and results:
This study explored the protective effect of genistein in reducing colitis caused by Salmonella infection. Salmonella caused colon inflammation through activating COX-2/PGE2 mediated by IκBα/NF-κB, and genistein inhibited colitis caused by Salmonella typhimurium infection. Salmonella infection increased colonic mucosal damage, Ki67 positive cells, and goblet cell loss, while the administration of genistein solved these pathological changes. In addition, it was further proved that Salmonella caused severe colitis related to goblet cell loss and activated the host crypt stem cells to repair the damaged epithelium through Wnt/β-catenin. Salmonella infection inhibited the host AKT/mTOR/p70S6K, activated STAT3/IL-22 and LC3-II pathways to induce autophagy to eliminate pathogenic bacteria. Genistein increased Lactobacillus in feces and reduced Salmonella colonization to inhibit colitis induced by Salmonella infection.
Conclusion:
This study demonstrated genistein alleviated colitis and inhibited the goblet cell loss caused by Salmonella infection through regulating the gut bacteria and intestinal stem cell development. This article is protected by copyright. All rights reserved.
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