Abstract
Scope:
Previous work reported that dietary supplementation with resveratrol lowers synovial hyperplasia, inflammatory markers and oxidative damage in an antigen-induced arthritis (AIA) model. Here, we investigated whether resveratrol can regulate the abnormal synovial proliferation by inducing autophagy and controlling the associated inflammatory response.
Methods and results:
Animals treated with resveratrol 8 weeks before AIA induction showed the highest significant signal for microtubule-associated protein 1 light chain 3 (LC3) by confocal microscopy. Besides, resveratrol significantly reduced p62 expression, but it did not increase the signal of beclin-1. Also, active caspase-3 expression, as well as PARP, were up-regulated in the AIA group, and were significantly reduced in resveratrol-treated AIA group. Likewise, resveratrol also mitigated Ang-1 and VEGF signals. Finally, resveratrol significantly reduced the serum levels of IL-1β, CRP and PGE2 , as well as NF-kB synovial tissue expression, which showed a significant correlation with p62 expression.
Conclusion:
Dietary supplementation with resveratrol induces the non-canonical autophagy pathway and limits the cross-talk existence with inflammation, which in consequence modulates the synovial hyperplasia. Preventive strategies that incorporate dietary intervention with resveratrol may offer a potential therapeutic alternative to drugs to influence the risk of RA and influence its course. This article is protected by copyright. All rights reserved.
Full text
