Abstract
Objectives:
This study evaluated the in vitro effect of different components of palm oil on enamel in a short-term erosive challenge.
Methods:
The acquired enamel pellicle (AEP) was previously formed in situ for 2 hours. Subsequently, the bovine enamel blocks were treated in vitro according to following solutions: G1 - palm oil; G2 - 85% tocotrienol solution; G3 - oily vitamin E; G4 - oily vitamin A; G5 -deionized water (negative control); G6 - stannous-containing solution (Elmex® Erosion Protection Dental Rinse) (positive control). After application of the treatment solutions (500 µl, 30 seconds), the blocks were immersed in 0.5% citric acid (pH 2.4) during 30 seconds (initial erosion). The response variable was the percentage of surface hardness loss. Data were analyzed by one-way ANOVA and Fisher's Test (p<0.05).
Results:
The positive control (G6), palm oil (G1) and oily vitamin E (G3) groups presented the lowest percentage of surface hardness loss, and were statistically different from the negative group (G5) (p<0.05), and no differences were found between these three groups. The 85% tocotrienol solution (G2) and oily vitamin A groups (G4) were not different to the negative control group.
Conclusions:
Stannous-containing positive control (Elmex® Erosion Protection), palm oil and oily Vitamin E were able to protect enamel against the erosive challenge performed in this in vitro study. In addition, vitamin E is probably the key ingredient of palm oil responsible for preventing enamel erosion.
Clinical significance:
Vitamin E presented similar preventive effect to a commercial mouthwash stannous-containing solution (Elmex® Erosion Protection) against initial erosion and, it can be considered as a promising natural alternative for the formulations of solutions aiming to prevent erosive tooth wear.
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