Abstract
Malignant melanoma is an aggressive cancer with a poor prognosis despite numerous advances in therapeutic strategies. Quercetin is a plant-derived flavonoid suggested to have potent anticancer properties. Quercetin has no demonstrable toxicity in humans, further supporting the possibility of using quercetin therapeutically. We chose to investigate quercetin efficacy against B16 murine melanoma cells and identify the mechanisms of anticancer activity. Treatment of B16 melanoma cells with 50 μg/mL quercetin resulted in a 75% reduction in viability from 6 through 48 h post-treatment. The reduction in cancer cell viability was comparable to or greater than what was observed with etoposide, an established chemotherapeutic. Specifically, we found Quercetin reduced the proliferation of B16 melanoma cells at 48 h as much or more than etoposide. Although quercetin reduced the proportion of cells in the S and G2/M stages of the cell cycle, this could largely be explained by an increase in the subG1 population in quercetin-treated cells (suggesting apoptosis). Quercetin-induced apoptosis was confirmed by flow cytometry analysis of Annexin V+ cells. Collectively, our findings demonstrate quercetin reduces proliferation and induces apoptosis of B16 melanoma cells in vitro.
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