Abstract
Previous studies indicate that dihydromyricetin (DHM) effectively improved glucose homeostasis and alleviated insulin resistance in population-intervened trials, yet the underlying mechanism remains obscure METHODS AND RESULTS: : Wild-type male mice and recombinase activating gene 1(Rag1)-/- mice (lacking adaptive immunity lymphocytes) were fed with control, high-fat diet (HFD), or HFD+DHM diets for 8 weeks. DHM effectively protected HFD feeding mice against hyperglycemia by promoting group 3 innate lymphoid cells (ILC3s) cells proliferation and interleukin 22 (IL-22) production. Furthermore, IL-22 secretion induced by DHM increased the expression levels of the tight junction (TJs) molecules to protect the intestinal barrier integrity, thereby decreasing the level of lipopolysaccharides (LPS), an endotoxin that is involved in the regulation of chronic tissue inflammation and insulin resistance. In addition, silent mating-type information regulation 2 homolog 3 (SIRT3) deficiency resulted in more serious obesity and intestinal barrier damage following HFD feeding and abolished DHM-mediated increase in IL-22 expression levels of ILC3 cells in SIRT3 knockout (SIRT3KO) mice. DHM reduced metabolic stress and enhanced mitochondrial respiratory capacity to promote cell proliferation and IL-22 secretion by activating SIRT3 in ILC3 cells CONCLUSIONS: : DHM improved IL-22 production of ILC3 cells and subsequently inhibited intestinal barrier dysfunction to alleviate hyperglycemia partially mediated by SIRT3.
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