Abstract
Background:
Bile duct ligation (BDL) has been used for evaluating the protective effects of different agents with anti-inflammatory and antioxidant properties against the liver and brain damages. Naringenin (N) and melatonin (M) were used as protectants in various models of diseases.
Aim:
In the current research, the combinational effects of these well-known anti-inflammatory and antioxidants agents were investigated against cerebral injuries induced by BDL in male rats.
Methods:
The animals were distributed into the following groups: Sham, BDL + Vehicle and BDL+ N + M. Neuronal damages were evaluated using biochemical, motor behavioral tasks and morphological assessments.
Results:
Based on the data, BDL resulted in the decreasing locomotor activity, which was reversed by N and M. Morphological study confirmed that BDL led to neurodegeneration in the cortex of the rats, and the N and M treatment preserved cortical neurons. In addition, immunohistochemical (IHC) study of the rat cortex showed that BDL resulted in increasing the activated astrocytes, and the N and M treatment reduced the number of activated cells.
Conclusion:
These results obviously depicted that combinational therapy with N and M had positive effects in the BDL rats, probably due to their synergistic anti-inflammatory and antioxidant activities.
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