Abstract
Background:
Human brain tumor glioblastoma (GBM) is the utmost hostile malignancy, currently lack of successful cure and deprived prognosis.
Objective:
To examine the anticancer effects of syringic acid (SA) on human cancer GBM cells.
Methodology:
The different doses of SA were added to GBM cells to fix its outcome on viability, invasion, relocation, apoptosis, and mRNA and protein levels. Hence, we explored the anti-proliferative, anti-invasive, and apoptotic activity of SA against GBM human U-251 cells.
Results:
MTT assay and live/dead assay revealed the anti-proliferative activity of SA on U-251 glioma cells. Apoptotic activity of SA was showed by DAPI staining, caspase-3, Bax, and Bcl-2 mRNA expressions. The cell cycle regulation was also confirmed by reducing the mRNA expression of cyclinD1, CDK4, and CDK6. Treatment of SA with U-251 cells suppressed MMPs expressions and enhanced TIMPs proteins levels.
Conclusion:
Our findings put forward that SA could prevent GBM cells invasion and relocation. SA is an ideal neuroprotective agent for controlling brain malignancy.
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