PubMed article abstract display

Vitamin B 12 supplementation influences methylation of genes associated with Type 2 diabetes and its intermediate traits

PMID: 29135286, Study year: 2018

Substance Condition
Vitamin B12👁 Diabetes Type 2👁
Linkhttps://pubmed.ncbi.nlm.nih.gov/29135286/
Abstract
Aim: To investigate the effect of B₁₂ and/or folic acid supplementation on genome-wide DNA methylation.

Methods: We performed Infinium HumanMethylation450 BeadChip (Zymo Research, CA, USA) assay in children supplemented with B₁₂ and/or folic acid (n = 12 in each group) and investigated the functional mechanism of selected differentially methylated loci.

Results: We noted significant methylation changes postsupplementation in B₁₂ (589 differentially methylated CpGs and 2892 regions) and B₁₂ + folic acid (169 differentially methylated CpGs and 3241 regions) groups. Type 2 diabetes-associated genes TCF7L2 and FTO; and a miRNA, miR21 were further investigated in another B₁₂-supplementation cohort. We also demonstrate that methylation influences miR21 expression and FTO, TCF7L2, CREBBP/CBP and SIRT1 are direct targets of miR21-3p.

Conclusion: B₁₂ supplementation influences regulation of several metabolically important Type 2 diabetes-associated genes through methylation of miR21. Hence, our study provides novel epigenetic explanation for the association between disordered one carbon metabolism and risk of adiposity, insulin resistance and diabetes and has translational potential.

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Updated: 2020-09-14