Abstract
Background:
Hydroxytyrosol is one of the phenolic compounds of olive oil and can induce anti-cancer effects on the colorectal cancer cells.
Objective:
The aim of the present study was to evaluate the free hydroxytyrosol and nano-capsulated hydroxytyrosol effects on the cell cycle arrest in HT-29 colorectal cancer cell line.
Methods:
The nano-capsulated hydroxytyrosol was synthesized in poly lactide-co-glycolide-co-polyacrylic acid (PLGA-PAA) copolymer. MTT assay was performed to evaluate the anti- proliferative and anti-tumor effects of the free hydroxytyrosol and nano-capsulated hydroxytyrosol. Finally, the relative expression of CDKN1A, CDKN1B and CCND1 genes was evaluated in the control and treated colorectal cancer cells by using Real-Time PCR.
Results:
The obtained results from the MTT assay showed that the cytotoxic effects of the nano-capsulated hydroxytyrosol on the colorectal cancer cell line (IC50= 6PPM) was significantly more than free hydroxytyrosol (IC50= 12PPM) after 72h. Also, nano-capsulated hydroxytyrosol showed more significant effects on the up-regulation of CDKN1A and CDKN1B genes, and down-regulation of the CCND1 gene in the colorectal cancer cells.
Conclusion:
In conclusion, the present study showed that the hydroxytyrosol led to die the colorectal cancer cell through the cell cycle arrest. Also, the PLGA-PAA copolymer dramatically caused to increase the cytotoxic effects of the hydroxytyrosol on the colorectal cancer cells.
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