Abstract
Background:
Quercetin (QCT) is a dietary flavonoid with many beneficial effects (e.g., antioxidant, antiaging, antidiabetic, antifungal effects, regulation of gastrointestinal motor activity in humans); furthermore, it induces apoptosis, cell cycle arrest, and differentiation.
Objective:
The apoptotic effects of OCT were investigated on SW480 human colon cancer cell lines in monolayer and spheroid cultures.
Methods:
Quercetin (40-200 μM) was applied, and inhibitory concentration (IC50) doses were determined for three-time intervals (24, 48, and 72 h). The effective dose was determined and applied for analyses, including staining with BrdU to investigate cell proliferation, terminal deoxynucleotidyl transferase dUTP nick, and labeling (TUNEL) to investigate apoptosis, and apoptosis-inducing factor (AIF) and Caspase-3 to investigate caspase-dependent or independent apoptotic pathways.
Results:
The effective dose of QCT was determined to be 200 μM and was found to induce apoptosis and inhibit cell proliferation at 24, 48, and 72 h, both in 2D and 3D cultures. Significant increases were observed in both caspase-3 and AIF staining, but cells showed greater caspase-3 staining compared with AIF staining at all time intervals (p<0.05).
Conclusion:
The QCT treatment groups showed more cell death and less cell growth than the untreated control groups in both 2D and 3D cultures of SW480 cell lines. The results suggest that quercetin induces apoptosis, inhibits cell proliferation, and has a protective role against colon cancer. However, further studies are needed to clarify its mechanism of action.
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