Abstract
Objective:
To analyze the clinical observation of salvianolic acid combined with panax notoginseng saponins combined with basic nursing intervention on cerebral ischemia-reperfusion injury in rats and its effects on the expression of apoptosis-related proteins Bcl-2, Bax and caspase-3.
Methods:
A total of 72 male Wistar rats were randomly divided into sham, ischemia/reperfusion (I/R), edaravone (Eda), salvianolic acid (SA), panax notoginseng saponins (PNS), and SA+PNS group. After administration for 5 days, the neurological function, cerebral infarction volume, brain index, and brain water content of rats were observed. ELISA kit assay was applied to measure the levels of IL-1β, IL-6, IL-8, TNF-α, MDA, SOD, GSH-Px, and T-AOC activity. Western blotting assay was used to detect the protein levels of p-53, NF-κB, Bcl-2, Bax, and Caspase-3 in the brain tissues surrounding infarction lesion.
Results:
Compared with sham group, the mNSS score, brain index, brain water content, infarction volumes, MDA activity, and the levels of IL-6, IL-8, TNF-α and IL-1β as well as the protein levels of p-53, NF-κB, Bax and Caspase-3 were significantly increased, while the levels of Bcl-2 protein, SOD, GSH-Px and T-AOC were significantly decreased in I/R group. However, these levels were reversed in SA group, PNS group and SA + PNS group. Moreover, these changes in SA + PNS group were more obvious than those in SA and PNS group, and the differences were statistically significant.
Conclusions:
SA, PNS and they combined with basic nursing have protective effects on cerebral I/R injury, and the combination with basic nursing has better effects than that used alone. The mechanism may be to regulate the expression of downstream apoptotic proteins by inhibiting the TLR4/NF-κB signaling pathway, thereby reducing neurological damage in rats.
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