Abstract
Background:
Breast cancer is the most common cause of deaths in women. The search for traditionally used medicinal plants which can serve as non-toxic and affordable anticancer drugs is the need of the hour. This study aimed to investigate the anticancer potential of extracts of L. coronopifolia against human breast carcinoma cell line (MDA-MB-321).
Methods:
The MDA-MB-231 cells were plated in 96 well plates and exposed to 10-1,000 μg/ml of L. coronopifolia for 24 h. The cytotoxic response of different extracts was measured by MTT assay, neutral red uptake (NRU) assay and cellular morphological alterations under the microscope.
Results:
A concentration-dependent decrease in the cell viability of MDA-MB-231 cells was observed after the exposure of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia. The cell viability was found to be 82%, 89% and 98% at 1000, 500 and 250 μg/ml, respectively in petroleum ether, 37%, 75% and 88% at 1,000, 500 and 250 μg/ml, respectively in ethyl acetate extract, 30%, 35% and 64% at 1,000, 500 and 250 μg/ml, respectively in chloroform extract and 44%, 65% and 82% at 1000, 500 and 250 μg/ml, respectively in ethanolic extract of L. coronopifolia exposed MDA-MB-231 cells. The results also exhibited morphological alterations in MDA-MB-231 cells exposed to various extracts. The cells treated with 250- 1000 μg/ml lost their original morphology and cell linkage as compared to control cells.
Conclusion:
These preliminary results suggest the promising anticancer potential of petroleum ether, ethyl acetate, chloroform, and ethanol extracts of L. coronopifolia against MDA-MB-321 cells. Further studies are required to know the mechanism(s) involved in the cell death.
Full text
