Abstract
Scope:
The consumption of green tea is considered to be associated with a lower incidence of neurodegenerative diseases. In the present study, we investigated the role of amyloid precursor protein cleavage, glial cell activation, neuroinflammation and synaptic alterations in the protective effects of green tea against the amyloid β (Aβ) accumulation and cognitive impairment.
Methods and results:
5XFAD mice were treated with green tea extract (GTE) for 8 or 16 weeks. Barnes maze and Y maze testing demonstrated that spatial learning and memory ability were markedly improved by GTE treatment. Immunofluorescence staining, ELISA and western blot showed GTE significantly alleviated the formation of Aβ and reduced the levels of sAPPβ and C99, as well as sAPPα and C83. Meanwhile, GTE suppressed GFAP and Iba1 levels in the glial cells, increased PSD95 and synaptophysin levels in synaptic cells. Further, the IL-1β level was decreased, RNA sequencing revealed the genes annotated in response to stimulus and immune response were regulated.
Conclusion:
Our findings indicated GTE suppresses Aβ levels and alleviates cognitive impairment in 5XFAD mice. These beneficial effects were accompanied by inhibition of APP cleavage pathways, suppression of glial cell activation and pro-inflammatory responses, and a reduction of synapse loss. This article is protected by copyright. All rights reserved.
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